Effects of electrical stimulation on the differentiation of mesenchymal stem cells into cardiomyocyte-like cells / 生物医学工程学杂志

The aim of this study is to investigate the effects of electrical stimulation ES) on the induction of rat bone marrow mesenchymal stem cells (rBMSCs) to differentiate into cardiomyocyte-like cells in vitro. The third or fourth-generation of the rBMSCs was randomly divided into three groups, i. e. ES group, 5-Azacytidine (5-Aza) group, and control group. Those in the ES group with complete medium were exposed to 1,2,4 and 6V, 2Hz, 5ms ES for 2h everyday,lasting for 10d. Those in the 5-Aza group were induced by 10 micromol/L 5-Aza for 24h, then the medium was changed to complete medium without 5-Aza. Those in the control group were only cultured with complete medium. The growth status and morphological features of rBMSCs were observed by inverted phase microscope. The mRNA expressions of GATA4, a-actin, ACTN2 and TNNT2 were determined by Real-time fluorescent quantification PCR, and the protein expression of TNNT2 was detected with immunofluorescence staining. The results showed that the mRNA expression level of the GATA4, a-actin, ACTN2 and TNNT2 and the protein expression level of the TNNT2 were significantly higher in the ES group and 5-Aza group, compared to those in the control group(P<0. 05). It suggested that ES could induce rBMSCs differentiation into cardiomyocyte-like cells in vitro.

Analysis of prognostic factors of the elderly patients with lacunar infarction / 中国基层医药

Objective To explore the correlation between the recurrence rate of the elderly patients with lacunar infarction and the risk factors including blood pressure, blood glucose, total cholesterol, triglycerides,low density lipoprotein cholesterol values, high-density lipoprotein cholesterol and other. Methods 207 over 60 years old patients with lacunar infarction occurred the first time were included in the study. All patients were followed up two years to assess the relapse rate and the risk factors of the patients with lacunar infarction. Results 207 cases of elderly patients with lacunar infarction complicated with hypertension,type 2 diabetes and hyperlipidemia were 148 cases,22 cases,7 cases respectively, and the recurrence rates of three groups were 8.78%, 18.18%, 14.29% respectively;The patients complicated with hypertension and 2 type diabetes were 6 cases,and the recurrence rate was 34.62%;The patients without hypertension,type 2 diabetes and hyperlipidemia were 4 cases,and the recurrence rate was 0%. In two years,27 cases of lacunar infarction recurred,and the recurrence rate was 13.04%. Conclusion Hypertension,type 2 diabetes,hyperlipidemia could also be considered as the high-risk predictor factors for the recurrence rate of the elderly patients with lacunar infarction.